Publications by authors named "V Sasidhar Manda"
The half-life of proteins is tightly regulated and underlies many cellular processes. It remains unclear the extent to which proteins are dynamically synthesized and degraded in different cell types and cell states. We introduce an improved DO labeling workflow and apply it to examine the landscape of protein turnover in pluripotent and differentiating human induced pluripotent stem cells (hiPSC).
View Article and Find Full Text PDFAim: This study aims to explore a sustainable and scalable approach using tomato fruit-derived sEVs (TsEVs) to deliver calcitriol for enhanced anticancer effects, addressing challenges of low yield and high costs associated with mammalian cell-derived sEVs.
Methods: TsEVs were isolated by centrifugation and ultrafiltration and characterized using DLS, TEM, and biochemical assays. Calcitriol was loaded into TsEVs via loading methods, with efficiency measured by spectrophotometry and HPLC.
Article Synopsis
- * Focus groups conducted with 19 women revealed that many had never heard of PrEP but showed a strong desire to learn more, highlighting barriers such as partner negotiation, adherence, and misconceptions about HIV.
- * Participants expressed high trust in family planning center providers and suggested that community outreach and tailored strategies are essential for improving PrEP uptake among these women.
Article Synopsis
- Surveillance of Transmitted Drug Resistance (TDR) is essential for understanding the changes in HIV trends, with a focus on multiple types of antiretroviral drugs and new treatments.
- A study involving 85 individuals diagnosed with primary HIV-1 infection (PHI) from 2020 to 2023 analyzed genetic sequences to identify TDR and its impact on drug resistance, revealing varying levels of resistance to several drug classes.
- The findings highlight the importance of monitoring TDR at the onset of HIV infection to improve treatment strategies and the effectiveness of pre-exposure prophylaxis (PrEP) with newer drug options.
In this study we examined the influence of hematopoietic stem cell-derived adipocytes (HSCDAs) on the proliferation and metastasis of high-grade serous carcinoma (HGSC) - the most common type of ovarian cancer. HSCDAs are a subtype of adipocytes that differentiate from myeloid precursors that traffic from bone marrow to adipose tissue and accumulate therein. These are distinct from conventional mesenchymal adipocytes (CMAs), which are derived from mesenchymal precursors.
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