[Modification of the sensitivity of Ehrlich ascitic carcinoma to apoptosis by transfection of thymocyte DNA].

We have demonstrated earlier that the replacement of the plasma membrane components of Erlich adenocarcinoma cells by the ones of thymocytes facilitates induced apoptosis in the tumor cells. Here we questioned whether similar effect can be achieved by transfection of thymocyte DNA. Lyposome transfer of normal DNA did not change the basal level of the programmed cell death.

[Modification of radiation sensitivity of lymphocytes of the rat thymus gland using cholesterol-enriched autoliposomes].

The influence of thymocyte enrichment with cholesterol on radiosensitivity of these cells has been studied. The enrichment was performed using autoliposomes. It was found that in the modified cells the processes of lipid peroxidation, enzymatic degradation of chromatin and apoptosis are inhibited.

[Comparative study, using fluorescent methods, of cell membranes and their reconstituted liposomes].

Cell plasma membranes and proteoliposomes reconstituted from solubilized plasma membranes of thymocytes and Ehrlich ascites carcinoma cells have been studied by fluorescent methods. It has been shown that proteoliposomes are characterized by greater polarization and rigidity of microsurroundings in membrane proteins and greater microviscosity of membrane lipids. Proteoliposomes from thymocyte membranes contain less membrane proteins and express lower polarity of the lipid bilayer than proteoliposomes from Ehrlich ascites cells.

[Effect of diuretics on electrolyte and non-electrolyte penetration of intercellular spaces].

The effect of novurit, mannitol and furosemide on permeability of the intercellular interstice of the frog urinary bladder by inulin, saccharose, iodide, lithium cation and fluorescein moving against a concentration gradient was examined. Novurit (0.5--6 mM/l) raised permeability of the intercellular interstice by all test substances and ions.