Publications by authors named "V S Kiroshka"
Most protocols to cryopreserve ovarian tissue utilize the permeable cryoprotective agents (CPAs) in 1.5 M concentration. However the issues related to the ability to use higher concentrations of CPAs have remained open.
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- Chitosan scaffolds are important in tissue engineering, but it's unclear how their microstructure affects cell interactions.
- The study focused on how chitin nano-whiskers (CNW) doped into chitosan matrices impact the behavior of rat bone marrow stromal cells (BMSCs) regarding adhesion, spreading, and proliferation.
- Results showed that BMSCs on highly porous, crystalline chitosan-CNW films behaved similarly to those on standard polystyrene plates, while amorphous structures reduced cell spreading and growth, indicating that the physical properties of the materials can be adjusted to influence cell behavior.
In this work it was investigated the dependence of the correlation of folliculogenesis and endocrine function of ovarian tissue on the degree of structural damage and oocyte volume changes during ischemia. It was shown that after 2 hours of ischemia at 37 °C the morphological transformation of the structural components of the ovarian tissue were reversible. In case of restoration of blood flow conditions by heterotopic transplantation estradiol level of animals was 25,9 ± 5,18 pg/ml, progesterone--18,48 ± 3,69 ng/ml, significantly higher than the castrated animals-recipients.
View Article and Find Full Text PDFWe analyzed the influence of initial properties of biopolymer fi lm extracellular matrices made of chitosan on adhesion of swine embryonic kidney epithelium-like cells, their morphology, growth, and proliferation. It was found that adhesion index of cells on the studied matrices was similar, but cell morphology had significant qualitative differences. Round non-flattened cells were described in endothelial cell cultures on chitosan-based matrices.
View Article and Find Full Text PDF[Dynamics of morphological and functional development of ovarian tissue allografts].
Fiziol Zh (1994)
June 2013
We examined the dynamics of folliculogenesis in ovarian tissue allograft and its endocrine function in an organism of castrated recipient rats during 100 days. Growth and development of ovarian tissue and its steroidogenic function in heterotopic transplantation were determined by its initial morphological organization. Allotransplantation of mature ovarian tissue was found to cause preservation of its morphological structure, whereas the transplantation of neonatal ovarian tissue caused formation of follicular cysts.
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