Modes of Escherichia coli Dps Interaction with DNA as Revealed by Atomic Force Microscopy.

Multifunctional protein Dps plays an important role in iron assimilation and a crucial role in bacterial genome packaging. Its monomers form dodecameric spherical particles accumulating ~400 molecules of oxidized iron ions within the protein cavity and applying a flexible N-terminal ends of each subunit for interaction with DNA. Deposition of iron is a well-studied process by which cells remove toxic Fe2+ ions from the genetic material and store them in an easily accessible form.

[Research on structural and functional characteristics (indicators) of apoptosis of human blood lymphocytes under the influence of carbon monoxide and ultraviolet (UV)-radiation].

The changes in the level of expression of membrane CD95 receptor, the structural state of DNA, parameters of biochemiluminescence of human blood lymphocytes under conditions of exposure to carbon monoxide (60-90 min) and UV-radiation (240-390 nm) at the doses of 151, 453 and 755 J/m2 have been investigated. The decrease in the level of expression of Fas-markers on the surface of immunocytes after incubation in the atmosphere at (60-90 min), the absence of changes in the DNA structure and the decreased intensity of lipid peroxidation processes in the cells were also found. It is established that UV-light (151-755 J/m2) exhibits a proapoptotic action, as evidenced by the increase in the expression of CD95 receptors on the surface of human blood lymphocytes and the decreased electrophoretic mobility of DNA in UV-irradiated cells.

[Micronucleus test of human oral buccal epithelium: problems, progress and prospects].

The articles by russian and foreign authors for the period from 2000 to 2012, devoted to the problems of application, analysis and interpretation of the results of micronucleus test in human buccal epithelium has been analyzed in the review. Nuclear abnormality founding in the cells of the oral mucosa has been described. The paper summarizes works devoted to the analysis of the influence of the micronucleus test methods (painting, taking scrapings) to its results.

[Apoptosis and necrosis of lymphocytes induced by UV-radiation in the presence of autological plasma].

The influence of UV-light (240-390 nm) in doses 151-3020 J/m2 on the nature of the death of lymphocytes cells of donor's blood (using markers of apoptotic and necrotic death of cells) and on the level of expression of the marker of apoptotic pre-preparation--CD95-receptor has been investigated. We have shown that UV-radiation increases expression of CD95-receptors which is caused mainly by de novo synthesis of the receptors. It has been revealed that during daily incubation of photo-modified lymphocytes (151 and 755 J/m2) without autological blood cell death occurs by receptor-involved apoptosis.

[Inulinases from various producers: the features of their permolecular organization].

The structural organization of inulinases from yeasts, fungi, and plants are researched. For studying their sizes, molecular weight, and permolecular organization, an approach consisting of a combination of atomic force microscopy with methods of dynamic light scattering, gel chromatography, and electrophoresis was used. It is shown that inulinases from Kluyveromyces marxianus and Aspergillus niger form geterodimers and inulinases from tubers of Helianthus tuberosus are present as both dimers and monomers.