First record of the presence of antibodies against Leptospira in Lagostomus maximus, and Leptospira weillii serogroup Celledoni in Chaetophractus villosus, Argentina.

Leptospirosis is a zoonosis with a worldwide distribution, which is transmitted through direct contact with infected animals or by contact with contaminated water or soil. Objectives was to examine the presence of antibodies against 23 serogroups of Leptospira in Lagostomus maximus and Chaetopractus villosus in La Pampa province, Argentina. Ninety-four serum samples from L.

Study on the interference of vaccine antibodies with the serological diagnosis of leptospirosis in cattle.

A simple IgG-specific ELISA for Leptospira spp. was compared with the microscopic agglutination test (MAT) to detect IgG antibody responses to a commercial vaccine in cattle. We used an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serovar copenhageni M 20.

Optimization and comparative analysis of LAMP and PCR techniques for the detection of leptospiral DNA in Golden Syrian hamsters.

Leptospirosis is a zoonotic disease with significant public health and economic impact worldwide. Rapid and accurate diagnosis is essential for effective prevention and treatment. This study optimized a loop-mediated isothermal amplification (LAMP) assay using BFo isothermal DNA polymerase with different colorimetric indicators.

Comparison of ELISA using recombinant LipL32 and sonicated antigen of leptospira for detecting bovine leptospirosis.

Leptospirosis is one of the most widely distributed zoonosis in the world. Bovine leptospirosis is a serious problem in bovine production, causing reproductive losses. The aim of this work was to compare recombinant LipL32 with sonicated antigen for detecting anti-Leptospira IgG antibodies in bovine serum using ELISA.

Use of the Leptospira sp. ligB C-terminus coding region as a diagnostic tool of animal leptospirosis.

Leptospirosis is the most widespread zoonosis worldwide, and it can cause reproductive failures in livestock, while in humans may vary from a mild fever to multi-organ failure and death. Due to this, in this study, we evaluated the usefulness of the segment encoding LigB C-terminus region, only present in pathogenic as target for a diagnostic PCR. This new PCR yielded a 100 % positivity for pathogenic Leptospira species and no cross-reactivity was found with intermediate or non-pathogenic species, or with other microorganisms, demostrating its high analytical specificity.