Publications by authors named "V R G Mercadante"
Here, we present a protocol for investigating the non-genetic heterogeneity of membrane proteins expression within murine muscle stem cell (MuSC) population isolated from injured skeletal muscles. We describe a protocol that employs flow cytometry technology to detect variations in membrane CRIPTO protein levels and ensure measurements standardization. We detail steps for muscle digestion, bulk muscle cell staining, and phenotypic analysis.
View Article and Find Full Text PDFMultiple administrations of bovine-appeasing substance during a 42-d preconditioning program followed by feedlot receiving and its effects on physiologic, health, and performance responses of feeder cattle.
J Anim Sci
January 2024
Article Synopsis
- The experiment assessed the impact of administering bovine-appeasing substances (BAS) to feeder cattle during a 42-day preconditioning phase followed by a feedlot period, focusing on their productivity and health.
- Ninety calves were transported and split into two treatment groups: one receiving multiple doses of BAS and the other a placebo, with various parameters like body weight and blood samples monitored throughout the program.
- Results showed no significant differences in daily gain or feed efficiency between the two groups, but the BAS group had lower plasma haptoglobin levels, indicating better health post-transport compared to the control group.
Article Synopsis
- Celiac disease (CD) is an autoimmune disorder triggered by gluten in genetically predisposed individuals, with a major treatment being a gluten-free diet (GFD).
- The study measured the expression of HLA DQ2.5 and TRAFD1 genes in CD patients, comparing those with active disease to those on GFD.
- Results showed no significant difference in HLA-DQ expression between the two groups, but TRAFD1 levels increased in patients on the GFD, suggesting it may help reduce gluten-induced inflammation.
A high incidence of pregnancy failures occurs in cattle during the second week of pregnancy as blastocysts transition into an elongated conceptus. This work explored whether interleukin-6 supplementation during in vitro embryo production would improve subsequent conceptus development. Bovine embryos were treated with 0 or 100 ng/mL recombinant bovine interleukin-6 beginning on day 5 post-fertilization.
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