[The reproduction of the hepatitis A virus in cell cultures].

A comparative analysis of reproduction of 9 strains of hepatitis A viruses (HSA-15, CF-979, MI, MBB 11/5, H-141, KMW-1, GBM, IH-26, WR-61) from different regions of the world in cell cultures (PLC/PRF/5, HEL-240, FRhK-4, MK) revealed the differences in the capacity of the viruses for reproduction in these cell lines. The factors influencing HAV reproduction in cell cultures such as temperature, medium, and sera were studied. In one-cycle infection, accumulation of vRNA and formation of virus particles were analysed.

[The adaptation of an isolate of the hepatitis A virus to reproduction in cell cultures].

A HAV strain derived from a patient in Moscow multiplied in a continuous cell line PLC/PRF/5 at 32 degrees C (variant MI-1) and at 37 degrees C (variant MI-1.1). These two variants of the strain MI retained the ability for reproduction both at 32 degrees C and 37 degrees C after passages in cell culture.

[Cultivation of hepatitis A virus in various cell lines and isolation of DNA sequences].

Using the MBB/11/5 strain originally adapted to the PLC/PRF/5 cell line, reproduction of the virus in diploid cells of human embryo fibroblasts, continuous primate lines (RAMT, FRhK-4) and human urinary bladder tumor (T-24) lines was studied. We obtained HAV DNA sequences (about 99% from vRNA) for the MBB/11/5 strain which were used as a probe for demonstration of vRNA synthesis in the infected cells.

[Analysis of ribonucleoproteins of influenza virus containing genomic and complementary RNA].

The density and sedimentation characteristics of ribonucleoproteins (RNP) containing genomic RNA from influenza virus and RNA complementary have been studied. Radioactive RNA from infected cells has been used for analysis. RNA classes of interest were isolated by reannealing with abundant nonradioactive genomic and complementary RNA and separation of resulting duplexes in electrophoresis.

[Synthesis of virus-specific and cellular proteins in the presence of bonafton].

Inhibition of the synthesis of virus-specific proteins of influenza A/WSN/33 virus in the cells of chick embryo fibroblasts and in continuous cells of dog embryonal kidney was discovered as was a negligible inhibition of the synthesis of cell proteins in the presence of an original synthetic antivirus drug bonaphthon. Experiments were made to attain selective inhibition of individual virus-specific proteins of influenza virus in these cultures. In model experiments in vitro in a cell-free protein-synthesizing system, bonaphthon was demonstrated to inhibit translation of RNA-virus of encephalomyocarditis on the virus template without affecting translation on the cell template of hemoglobin mRNA.