Derivatives of benzo(c)fluorene. XI. Effect of Benfluron on hemopoietic stem cells.

The effect of Benfluron-- 5-(2-N,N-dimethylamino-ethoxy)-7-oxo-7H-benzo(c)fluorene hydrochloride--on hemopoietic stem cells was tested by the production of spleen cell colonies (CFU-S) in irradiated mice following application of bone marrow cells and by the production of hemopoietic colonies (CFU-C) in semisolid agar. The reduced numbers of CFU-S were found in mice applied Benfluron or Benfluron-treated bone marrow cells. The increased numbers of CFU-S, however, were found in mice receiving bone marrow cells from Benfluron-treated donors.

Derivatives of benzo(c)fluorene. X. Inhibitory effect of Benfluron on cellular immunity.

The effect of a cytostatic drug Benfluron-- 5(2-N,N-dimethyl-amino-ethoxy)-7-oxo-7H-benzo(c)fluorene hydrochloride was tested in mice on skin graft survival, graft-versus-host reaction (GVHR) and for mitogenic stimulation of human lymphocytes. Application of Benfluron resulted in a prolonged skin graft survival. The regional and systemic GVHR was potentially inhibited by p.

Antileukemic effects of 5-(2-dimethylaminoethoxy)-7-oxo-7H-benzo(c) fluorene, substance VUFB 13 468, on L1210 and La leukemia.

The antineoplastic effect of 5-(2-dimethyl-aminoethoxy)-7-oxo-7H-benzo(c) fluorene, substance VUFB 13 468, was studied in leukemia L1210 and leukemia La. It was found that the test substance, in the form of base and in the form of hydrochloride, influenced favorably the survival of leukemic mice, in which the life span increased in dependence on the dose and the scheme of its application.

The effect of Damvar on the formation of hemopoietic splenic colonies in mice, and on the peripheral blood picture in rats and dogs.

The present paper deals with the effect of Damvar on hemopoiesis. The test of formation of hemopoietic splenic colonies (CFU-S) was used in mice, the peripheral blood picture and bone marrow differential after long-term administration of Damvar was tested in rats and dogs. The bone marrow cells from mice after a single dose of Damvar or cells incubated with Damvar in vitro showed no reduction of colony-forming activity.

Combined therapy of L1210 leukemia with Damvar and cytostatics.

Damvar, the delta-(2-amino-6-hydroxy-3,4-dihydro-4-oxo-5-pyrimidinyl) valeric acid, administered alone s. c. or i.