A novel translational bioinformatics framework for facilitating multimodal data analyses in preclinical models of neurological injury.

Pediatric neurological injury and disease is a critical public health issue due to increasing rates of survival from primary injuries (e.g., cardiac arrest, traumatic brain injury) and a lack of monitoring technologies and therapeutics for treatment of secondary neurological injury.

Special series on Impact of Real-Life Environmental Exposures on Reproduction.

Environmental exposures to endocrine disrupting chemicals (EDCs), air pollution, biosolids, fracking materials, some pharmaceutical agents, and heat stress adversely affect reproductive health and exert long-ranging effects on maternal, paternal, and child health (Gore et al. 2015; Brehm & Flaws 2019; Gonsioroski et al. 2020; Peretz et al.

Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies.

To distinguish DNA methylation (DNAm) from cell proportion changes in whole placental villous tissue research, we developed a robust cell type-specific DNAm reference to estimate cell composition. We collated new and existing cell type DNAm profiles quantified via Illumina EPIC or 450k microarrays. To estimate cell composition, we deconvoluted whole placental samples ( = 36) with robust partial correlation based on the top 30 hyper- and hypomethylated sites identified per cell type.

Reproductive Health in Trans and Gender Diverse Patients.

Worldwide, approximately 4.5% of adults identify as transgender or gender diverse (TGD), with up to 8.4% of youth identifying as TGD (Scheim et al 2024), suggesting that the number of TGD patients reaching reproductive age is increasing.

Characterizing DNA Methylation and Hydroxymethylation in Cord Blood and Identifying Sex-Specific Differences using the Illumina EPIC Array.

DNA methylation, an epigenetic mark, has become a common outcome in epidemiological studies with the aid of affordable and reliable technologies. Yet the most widespread technique used to assess methylation, bisulfite conversion, does not allow for the differentiation of regular DNA methylation (5-mC) and other cytosine modifications, like that of hydroxymethylation (5-hmC). As both 5-mC and 5-hmC have distinct biological roles, sometimes with opposing effects, it is crucial to understand the difference between these marks.