Effect of 6-azacytidine on the course of experimental adenoviral infection in newborn Syrian hamsters.

Adenoviral infection is a serious human pathology leading to respiratory, gastrointestinal and ocular disorders and epidemic outbreaks, especially in children's groups. Here we present the results from an investigation of anti- adenoviral effect of 6-azacytidine (6-AC) both in vitro and in vivo. The selectivity index of 6-AC for adenovirus type 5 in HEp-2 cells was 374, the 50% effective concentration was 0.

[Evaluation of metabolic parameters in vitro as a model for testing the cytotoxicity of antiviral drugs].

A new test system based on in vitro assessment of the cellular viability and/or metabolism is proposed, which offers an informative approach to the screening of antiviral drugs. Cytotoxic effects of the antiviral drugs rimantadine and polyrem were studied on the cultures of some mammalian cells. The results of short-term (2 h) exposures with the drugs tested were close to their LD50 values in mammals, which makes the proposed test system promising for the assessment of drug toxicity in vivo for the whole organism.

[Protective effect of cycloferon in experimental influenza].

Protective effect of granulated cycloferon has been studied in albino mice infected with influenza. The drug induced interferon production and prolonged the life span, decreased the mortality, suppressed the virus reproduction in the lungs, and decreased the infective activity of the virus. Morphologically the drug decreased the intensity of viral lesions in the bronchiolar epithelium, impeded the infection dissemination, and stimulated the productive (cellular) component of local inflammatory reaction.

[Intracellular localization of cycloferon, its binding with DNA and stimulation of cytokines expression after exposure to cycloferon].

Despite the wide usage of immunomodulating preparates including interferon inducers in medical practice little is known about their mechanism of action. We investigated some theoretical aspects of action of potent interferon inducer--cycloferon (10-carboxymethyl-9-acridanone), such as intracellular localization, ability to DNA binding and cytokine expression stimulation. This preparate has been found to be localized in nuclei of monocytic cells U-937, T- and B-lymphocytes and HeLa cells.