Displacement effect during HPLC preparative purification of human insulin.

HPLC plays a key role in the preparative purification of human insulin. A21-desamidoinsulin is one of the impurities that possesses the chromatographic behavior similar to that of insulin and hence separation from this by-product is rather difficult at the process scale. During the optimization of insulin reversed-phase HPLC purification, when a column was sufficiently overloaded, the effect of displacement of A21-desamidoinsulin molecules from active groups of sorbent by insulin ones was observed.

[Functional properties of B-lymphocytes of patients with a B-cell variant of chronic lymphocytic leukemia and chronic glomerulonephritis].

The common features of the pathogenesis of chronic lymphocytic leukemia (CLL) and chronic glomerulonephritis (CGN) have been shown as a result of a comprehensive study of the functional properties of B cells. Both CLL and CGN are accompanied by the appearance and accumulation of different types of activated B cells characterized by high spontaneous proliferation and/or Ig secretion; or sensitivity to T cell growth and differentiation factors; or capacity for Ig secretion; in mitomycin C treated B cell cultures (i.e.

[A clinico-pathogenetic approach to choosing the therapeutic procedure in chronic glomerulonephritis].

The results have been compared of the use of two schedules (continuous and cyclic) of multimodality therapy of chronic glomerulonephritis (CGN) including cytostatics, corticosteroids, anticoagulants and deaggregation agents. Continuous therapy was carried out for 12.9 +/- 0.

[Clinico-pathogenetic characteristics of hematuric glomerulonephritis].

Criteria for the diagnosis of hematuric nephritis (HemN) are proposed. Based on these criteria a group of patients (n = 157) was formed. As a result of the observation of many years, the main variants of HemN were characterized and its etiological polymorphism was demonstrated.

[Precursor thymus-dependent lymphocytes in variants of chronic glomerulonephritis].

Altogether 67 patients with chronic glomerulonephritis (CGN) were examined for the content of thymus-dependent precursor lymphocytes (auto-RFC, pre-T-cells). The patients suffering from CGN were characterized by the high content of the young post-thymic T-cells (auto-RFC) and high affinity T lymphocytes carrying a receptor for the Fc fragment of IgM as compared to normal donors. In patients with different clinical patterns of CGN, an analysis was made of the content of precursor thymus-dependent lymphocytes.