Expression of immunomodulator gene mRNA in co-culture of mesenchymal stromal cells from the placenta and human mononuclear blood cells.

The expression of mRNA of cytokines and immunoregulatory molecules characterizing the interaction of mesenchymal stromal cells from chorionic villi of postpartum placenta and allogenic mononuclear blood cells was studied during 3-day co-culturing of these cells. The expression of foxp3, il2ra, and il10 mRNA in floating mononuclear cells increased from day 1 to 3 in co-culture, which can refl ect the process of induction of regulatory T cells in the lymphocyte population under the action of mesenchymal stromal cells.

Comparison of the expression of immunomodulatory factors in cultures of mesenchymal stromal cells from human extraembryonic tissues.

mRNA of genes (tgfβ, il6, il10, il1β, and vegf165) involved in cell proliferation, inflammatory, anti-inflammatory, and angiogenic processes were detected and quantified in cultures of mesenchymal stromal cells of different passages derived from human extraembryonic tissues (amniotic sac, umbilical cord, chorionic villi and trophoblast of the placenta). The concentrations of IL-10, IL-6, IL-1β, and TGFβ were measured.

Isolation of mesenchymal stromal cells from extraembryonic tissues and their characteristics.

We describe a method of isolation of human mesenchymal stromal cells from the umbilical cord (Wharton's jelly) and human placenta: amnion, placental villi, and trophoblast. Morphology, immunophenotypic characteristics, and differentiation potencies of isolated cells were studied. The capacity of mesenchymal stromal cells from extraembryonic tissues to osteogenic, adipogenic, and chondrogenic differentiation was demonstrated and the dynamics of this process was described.

Experimental study of corrective effects of human umbilical blood nuclears under conditions of a posthemorrhagic state.

Intravenous transfusion of nucleated cells from human umbilical cord blood (10(6)/ml saline) to female rats 3 h after massive blood loss produced a pronounced corrective effect (day 5): alleviates posthemorrhagic changes and normalizes hemoconcentration parameters, metabolic disorders, and behavioral reactions.

Immunomorphological characteristics of endometrium in women with chronic endometritis.

The number of monocytes, macrophages (CD14+), and large granular lymphocytes (CD56+) in the endometrium increased in chronic endometritis. These changes are an unfavorable prognositic signs for the onset of pregnancy. Combination therapy improved the phenotypic composition of endometrial cells.