[Antioxidant properties of apelin-12 and its structural analogue in experimental ischemia and reperfusion].

Effects of apelin-12 H-Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Met-Pro-Phe-OH (A12) and its modified analogue H-(NMe)Arg-Pro-Arg-Leu-Ser-His-Lys-Gly-Pro-Nle-Pro-Phe-OH (I) on activity of antioxidant enzymes, formation of malonic dialdehyde (MDA) and generation of reactive oxygen species (ROS) were studied in ex vivo and in vivo models of myocardial ischemia and reperfusion (I/R) injury in Wistar rats. Preischemic infusion of peptide A12 or AI enhanced cardiac function recovery of isolated perfused heart and was accompanied by a marked attenuation of ROS generation detected by electron paramagnetic resonance (EPR) technique in myocardial effluent at early reperfusion compared with control. Intravenous administration (i.

[The influence of inhibiting no formation on metabolic recovery of ischemic rat heart by apelin-12].

Apelin 12 (A-12) was synthesized by the automatic solid phase method with use of Fmoc 1H-NMR spectroscopy and mass spectrometry. Effects of apelin-12 (a peptide comprised of 12 aminoacids, A-12) on recovery of energy metabolism and cardiac function were studied in isolated working rat hearts perfused with Krebs buffer (KB) containing 11 mM glucose that were subjected to global ischemia and reperfusion. A short-term infusion of microM 140 A-12 in KB prior to ischemia enhanced myocardial ATP, the total adenine nucleotide pool (SigmaAN = ATP + ADP + AMP) and the energy charge of cardiomyocites ((ATP + 0.

[Synthesis and cardioprotective properties of apelin-12 and its structural analogs].

The apelin-12 and a number of its analogs, resistant to degradation of proteases, were synthesized by Fmoc- method of SPPS. By-products of synthesis were examined. It was found that serine hydroxyl group was sulfating during the final deprotection of apelin-12 (I) and its analogs.

[Effects of dinitrosyl iron complex with glutathione and its components on ischemic rat heart during reperfusion].

The effects of the drug, a complex of dinitrosyl iron with glutathione lyophilized on dextrane, and its components: glutathione, nitrosoglutathione, dextrane, as well as nitric oxide released from dinitrosyl iron with glutathione--on the energy metabolism and functional recovery of isolated perfused rat heart subjected to global ischemia and reperfusion have been studied. The infusion of 100 nM dinitrosyl iron with glutathione after ischemia substantially enhanced the recovery of coronary flow, the cardiac contractile and pump functions during reperfusion with simultaneous preservation of myocardial high-energy phosphates, and cell membrane integrity. It was shown by the EPR method that these effects were associated with the transfer of Fe+(NO+)2 groups from dinitrosyl iron with glutathione to thiol-containing proteins of cardiomyocytes and coronary vessels.

[Effects of dinitrosyl iron complex on metabolism and cellular membranes in ischemic rat heart].

Effects of exogenous NO donor--dinitrosyl iron complex with reduced glutathione (DNIC-GS) on functional recovery of isolated perfused rat heart subjected to global ischemia and reperfusion have been studied. DNIC-GS administration after ischemia substantially improved contractile and pump function recovery within a concentration range of 34 nM - 5 uM. In case of DNIC-GS administration before ischemia a two-phase influence was found--cardioprotective action for 67 nM and damaging one for 250 nM.