Publications by authors named "V N Pivovarov"

Immuno-PCR (iPCR) is one of the methods used for the detection of a wide range of analytes and features the high sensitivity of the polymerase chain reaction (PCR) method. iPCR uses antibodies coupled to DNA, followed by the amplification of the attached DNA using RT-PCR. Two major types of antibody-DNA conjugates are currently used, which are obtained as a result of non-covalent (biotin-streptavidin) or covalent interactions.

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Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein-Barr virus (EBV) in the human blood serum.

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Aim: Estimation of specific IgE is essential for the prevention of allergy progression. Quantitative immuno-PCR (qiPCR) can increase the sensitivity of IgE detection. We aimed to develop qiPCR and compare it to the conventional ELISA in identification of IgE to Alt a 1 and Fel d 1 allergens.

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Article Synopsis
  • The review highlights the content and breeding of vegetable crops that are rich in biologically active substances beneficial for functional nutrition.
  • Research showcases specific vegetable varieties, like onions with high flavonoid content and eggplants containing anthocyanins, emphasizing their health properties.
  • Notable findings include hot peppers as excellent sources of vitamin C and capsicum, as well as pumpkin and tomato varieties known for their carotenoid and lycopene content, respectively, underscoring their antioxidant potential.
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