[Transplantation of stem/progenitor cells of human brain into the brain of adult rats].

We studied the development of stem/progenitor cells of the human brain transplanted in the adult rat brain after reproduction in an in vitro tissue culture. It was preliminarily shown by the immunological methods that the stem cells grown in a medium with growth factors formed neurospheres, which were heterogenous and contained both stem and progenitor cells of the human brain. The cells were implanted in the hippocampus, striatum, or lateral ventricle of the rat brain as a suspension or aggregates (neurospheres) and their behavior and differentiation were studies within 10, 20, and 30 days using the morphological and immunochemical methods.

[The DNA content of the neurons in the embryonic cerebellum transplanted into the cerebral cortex of adult rats].

Cerebellum of rat embryos at day 17 of gestation was transplanted into sensomotor cortex of adult syngenic rats. DNA content in granule and Purkinje cells of the transplant was determined cytophotometrically on day 30 after surgery. It is shown that granule cells are diploid; about 3% Purkinje cells contain hyperdiploid and tetraploid nuclei which corresponds to the content of such cells in the adult cerebellum.

The recovery of the ultrastructure of hyperchromic host neurons under the influence of fetal neurotransplants.

Fetal neocortical transplants placed into a previously prepared cavity in the cortex of adult animals inhibited destructive processes in host neurons surrounding the place of injury. This was demonstrated by a gradual reduction in the number of hyperchromic and shrunken host neurons and the recovery of the ultrastructure of dystrophic cells which die as a result of neuronophagia or sclerosis in the absence of the transplants.