[Diagnostic bacteriophage V32 as a tool for the rapid identification of Escherichia coli serogroup O].

Bacteriophage V32, a representative of bacterial viruses of the Myoviridae family Ounavirinae subfamily, is proposed for search and identification of E. coli O serogroup, including Shiga-toxin producing E. coli O:H (STEC O:H), among cultures of enterobacteria from the primary seeding of the material studied.

[Molecular-genetic characterization of shiga-toxin producing Escherichia coli isolated during a food-borne outbreak in St. Petersburg in 2013].

Unlabelled: Shiga toxin-producing Escherichia coli (STEC) food-borne infections are reported worldwide and represent a serious problem for public healthcare. In the Russian Federation there is little information on epidemiology and etiology of STEC-infections as well as on molecular-genetic peculiarities of STEC pathogens.

Objective: Our aim was to describe a food-borne outbreak as hemorrhagic colitis (HC) along with hemolytic uremic syndrome (HUS), enterocolitis, and acute gastroenteritis in children in St.

[Cause of abnormal acidity of lysozyme ionogenic active center groups at cell wall lysis].

pH-Dependence of the kinetic parameters of Micrococcus lysodeicticus cell lysis under the action of the protein hen egg lysozyme at the pH 6.9-10.0 at 25 and 37 degrees C has been investigated.

Adsorption chromatography of proteins.

A method for adsorption chromatography of proteins is proposed. A protein solution is passed through a cellulose column at a pH value corresponding to an isoelectric point of the protein. Depending on the charge of unwanted proteins, they either remain at the origin (if charges of protein and ion-exchanger are opposite) or are released from the column (if charges of protein and ion-exchanger coincide).

Diverse antimicrobial killing by Enterococcus faecium E 50-52 bacteriocin.

An effective bacteriocin was identified and characterized. Lactic acid bacteria were screened against Campylobacter jejuni. One bacteriocin producer, Enterococcus faecium (NRRL B-30746), was studied.