[Aggregates of islet cells, their structure and insulin-producing activity during cultivation in vitro].

Purified rat islets were dissociated into single-cell suspension with an EDTA-Trypsin treatment. During a stationary culture in vitro the islet cells reassociated forming aggregates (neoislets). Electron microscopy revealed that the aggregates consisted mostly of beta-cells and not numerous alpha-cells.

[Some characteristics of the histological structure of the fetal bovine pancreas].

Some properties of histological structure of fetal bovine pancreas were demonstrated using light microscopic methods. The different forms of acino-insular complexes were described: 1) acino-insular complexes with single B-cells including epithelial layer of acini; 2) acino-insular complexes with segmental (sector) localization of insular cell groups; 3) acino-insular complexes with small and more large groups of endocrine cells timely contacted with acini; 4) acino-insular complexes at the stage of separation of endocrine cell groups (microislets) from acini. The consideration of acino-insular complexes in morphogenesis of bovine endocrine pancreas in discussed.

[Effect of pancreatic islet cell culture allotransplantation on the clinical course of proliferative diabetic retinopathy].

The authors describe the effect of intramuscular human fetal pancreatic islet cell (IC) culture transplantation on the clinical course of proliferative diabetic retinopathy (PDR). Four patients with the preterminal stage of PDR were followed-up no less than 2 yrs. after transplantation.

[Preparation of liver cultures from human embryos].

The cultures were obtained from 9-12-week-old human embryonic liver. 24-36 hours after seeding the cultures containing a multilayer focus of adhesion and a monolayer growth zone were formed. The growth zone contained hepatocytes with bile pigment granules in the cytoplasm.

[Transplantation angiopathies and vasculitis after allogeneic grafting of cadaver kidney to humans].

The material of 560 allogenic corpse kidney transplantations was studied and the conclusion drawn on the possibility of the development in the kidney graft of two types of vascular lesions. The first type is referred to as a genuine angiopathy the causes and structure of which change depending on the time of donor kidney persistence in the recipient. The second type is regarded as immune vasculitis.