[Monoclonal antibodies cross-reacting with fibroblasts of interstitial connective tissue of the myocardium and cell wall protein antigens of group A Streptococcus].

Monoclonal antibodies (MCA) B6/5 and C5/3 were obtained after immunization of BALB/c mice with the protein non-type-specific antigens (NTSA) of streptococcal group A cell wall. MCA B6/5 in the indirect immunofluorescence react with human and animal interstitial connective tissue (ICT) of the myocardium and human fibroblast culture cells. MCA C5/3 react with the bands of muscle fibers of the myocardium.

[Monoclonal autoantibodies to different epithelial structures of the thymus gland obtained by the immunization with streptococcal group A antigens].

By the indirect immunofluorescence method it was shown to which epithelial thymus structures monoclonal antibodies (mAT) reacting with the different epidermal structures are directed. These mAT related to the autoantibodies were obtained earlier, as a result of lymphoid cells polyclonal activation, by the immunization of BALB/c mice with streptococcal group A nonspecific protein antigens of the cell wall. It was shown that mAT A6/1, reacting with the basal layer of the skin epithelium are directed to the epithelium of the cortical and medullar thymus zones, which is regarded as the so called endocrinal epithelium.

[The localization of structurally specific antigens in squamous cell skin cancer using monoclonal antibodies to epidermal antigens].

Immunomorphological studies by indirect immunofluorescent method using monoclonal antibodies to antigens of basal cells, suprabasal cells and basal membrane of the epidermis were carried out in 8 cases of squamous-cell skin cancer. Specific characteristics of localization of structure-specific antigens (as compared with normal human skin) in squamous-cell skin cancer are discussed.

[Production of monoclonal antibodies to tissue-specific antigens of human skin epithelium].

Immunization of BALB/c mice with non-type-specific protein antigens of the cellular wall of group A streptococcus and formalin-treated streptococcal culture resulted in stimulating production of polyclonal autoantibodies to antigens of the epithelium of human and murine skin. As a result of hybridoma technique using splenic cells of immunized animals, monoclonal antibodies to different antigens of epidermal cell cytoplasm, i.e.

[Reaction of monoclonal antibodies A 6/1 with the antigen determinant of ultrastructures of tumor cells in epidermal-type tegmental tissues].

Using indirect immunoenzyme assay and ultrathin sections, it was shown that antibodies against basal cell antigens react with tonofibril and desmosome filaments of squamous tissue carcinoma cells.