Production of thermozymocidin (myriocin) by the pyrenomycete Melanconis flavovirens.

Submerged culture of the pyrenomycete Melanconis flavovirens produces a strongly active antifungal antibiotic. The antibiotic was isolated from the culture mash. In purified compound the physico-chemical characteristics, including 1H NMR spectrum, were estimated.

Flavovirin--a new antifungal antibiotic produced by the pyrenomycete Melanconis flavovirens.

The pyrenomycete Melanconis flavovirens was found to produce a mixture of two antifungal antibiotics. The first has been already described; the antibiotic is identical with thermozymocidin (myriocin). The second antibiotic is a new compound, whose isolation and identification is presented in this paper.

Glucose-2-oxidase activity in mycelial cultures of basidiomycetes.

The activity of intracellular glucose-2-oxidase was tested in 40 species of 26 basidiomycete genera. The enzyme catalyzing the oxidation of D-glucose to the dicarbonyl sugar D-arabino-2-hexosulose was demonstrated in mycelial extracts of 9 species of Aphyllophorales and 6 species of Agaricales cultivated in liquid media. In the majority of species exhibiting this activity hexosulose was detected in the cultivation medium.

Effect of glucitol on the production of mucidin in Oudemansiella mucida.

When studying the biosynthesis of mucidin under production (glucose as the main carbon source) and non-production (glucitol as the main carbon source) conditions it could be shown that the producer, Oudemansiella mucida, utilizes glucitol both for growth and for mucidin biosynthesis. However, the production of mucidin is 10 times lower than on glucose. When the culture was preincubated on glucose and transferred to non-production conditions the negative effect of glucitol could not be demonstrated.

Metabolism of aromatic acids in the antibiotic-producing basidiomycete Oudemansiella mucida.

Aromatic acids were determined in the mycelium and fermentation medium of Oudemansiella mucida. Coumaric acids (both m- and p-), p-hydroxybenzoic acid (salicylic acid) and benzoic acid were found to predominate in the mycelium. Phenylacetic acid represents the main component in the medium.