[Influence of siRNA complexes on the reproduction of influenza A virus (Orthomyxoviridae: ) ].

Introduction: Influenza is one of the most pressing global health problems. Despite the wide range of available anti-influenza drugs, the viral drug resistance is an increasing concern and requires the search for new approaches to overcome it. A promising solution is the development of drugs with action that is based on the inhibition of the activity of cellular genes through RNA interference.

Evaluation of Epigenetic Age Based on DNA Methylation Analysis of Several CpG Sites in Ukrainian Population.

Epigenetic clocks are the models, which use CpG methylation levels for the age prediction of an organism. Although there were several epigenetic clocks developed there is a demand for development and evaluation of the relatively accurate and sensitive epigenetic clocks that can be used for routine research purposes. In this study, we evaluated two epigenetic clock models based on the 4 CpG sites and 2 CpG sites in the human genome using the pyrosequencing method for their methylation level estimation.

Biodistribution analysis of cisplatin in liposomal form in animals with cisplatin-resistant and cisplatin-sensitive carcinoma.

Aim: To analyze the relation between pharmacokinetics of cisplatin in liposomal form and antitumor efficacy toward cisplatin-resistant and cisplatin-sensitive variants of Guerin carcinoma.

Methods: Concentration of platinum was measured by atomic absorption spectrophotometry (C115M1 "Selmi", Ukraine). Elimination constant was calculated based on the dynamics of cisplatin concentration in time period between 1 h to 24 h using nonlinear regression analysis.

[Functional activity of metal contained enzymes with antioxidant activity in blood of patients with stomach cancer and during the use of anticancer autovaccine].

The authors presented in the article results of assessment of metal contained enzymes with antioxidant activity, ceruloplasmin (CP) in plasma blood, Cu-Zn superoxide dismutase (SOD) and catalase in red blood cells of patients with I-IV stage stomach cancer during surgical treatment and application of anticancer autovaccine (AAV) to prevent relapses and methastases. It was revealed compared with the donors that at stages of the treatment, before operation and after resuscitation, the level of activity of CP was higher in 1,3 times and in 3 times was higher the level of SOD. The change of catalase activity was within the limits of physiological values.

The study of possibility to elevate antitumor activity and decrease of systemic toxic effects of cisplatin by its binding with deliganded albumin.

Aim: To evaluate antitumor and toxic action of cisplatin (CP) in non-bound form and in a complex with deliganded albumin.

Methods: To study complex-formation between CP and albumin, differential scanning and isothermic flow microcalorimetry were used. For quantitive evaluation of albumin-bound CP, the method of ultrafiltration was applied.