Publications by authors named "V Micheli"
Article Synopsis
- Next-generation sequencing (NGS) is replacing Sanger sequencing for HIV genotypic drug resistance testing, and this study assessed how consistent different interpretation tools are in a real-world setting.
- The research involved analyzing NGS results from 11 Italian labs using the AD4SEQ HIV-1 Solution v2 kit and compared interpretations from SmartVir, HyDRA Web, and Stanford HIVdb.
- Findings indicated that viremia levels and HIV subtypes influence the validity of NGS-GRT, with only mutations present at frequencies over 10% showing reliable agreement among the interpretation tools.
The association between human papillomavirus (HPV) and other sexually transmitted infections (STIs) in anal lesions still remains unclear. Aim of the study was to evaluate the prevalence of simultaneous infection of HPV and Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis in individuals screened for HPV anal infection. A total of 507 anal samples were tested for both anal HPV and STIs: 16% resulted positive for one or more non-HPV STIs.
View Article and Find Full Text PDFBackground: Mpox virus (MPXV) has recently spread outside of sub-Saharan Africa. This large multicentre study was conducted in Lombardy, the most densely populated Italian region accounting for more than 40% of Italian cases. The present study aims to: i) evaluate the presence and the shedding duration of MPXV DNA in different body compartments correlating the MPXV viability with the time to onset of symptoms; ii) provide evidence of MPXV persistence in different body compartment as a source of infection and iii) characterize the MPXV evolution by whole genome sequencing (WGS) during the outbreak occurred in Italy.
View Article and Find Full Text PDFArticle Synopsis
- - The study assessed how the V106 polymorphism affects the effectiveness of doravirine, a medication for HIV, finding low prevalence rates of the V106I mutation, particularly higher in subtypes D and F.
- - Different mutations (V106A, V106M, and Y188L) showed varying impacts on doravirine susceptibility, with V106I having a minimal effect in site-directed mutants and most tested clinical isolates remaining susceptible.
- - Although the V106I mutation's prevalence is low, particularly regarding its impact on doravirine resistance in subtype F1, further research is needed to understand the clinical implications.
The Patient Preference Survey aims to understand unmet needs related to riluzole management in people with Amyotrophic Lateral Sclerosis (ALS) and to identify which characteristics of a new formulation could better match their preferences. The survey involved 117 people with ALS (PALS) treated with riluzole in four European countries. The dysphagic PALS were least satisfied with the riluzole tablet and oral suspension and with ease in self-administration; up to 68% of respondents postponed or missed the treatment due to swallowing difficulties and need of caregiver assistance.
View Article and Find Full Text PDF