Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.

In eukaryotes, nucleosomes form a barrier to DNA templated reactions and must be dynamically disrupted to provide access to the genome. During nucleosome (re)assembly, histones can be replaced by new histones, erasing post-translational modifications. Measuring histone turnover in mammalian cells has mostly relied on inducible overexpression of histones, which may influence and distort natural histone deposition rates.

Amino acid utilization and isotope discrimination of amino nitrogen in nitrogen metabolism of rat liver in vivo.

Urea and plasma protein differ in natural 15N abundance up to 10%. The origin of this difference is the branched nitrogen metabolism in the liver. One main branch is the protein synthesis pathway, the other the urea synthesis pathway.

Variable temperature UHV dual-range cell for spectroscopic surface studies.

A stainless steel cell for transmission spectroscopy of surface compounds is described. This cell allows subsequent spectra recording of the sample in two spectral regions requiring different window materials. The sample temperature can be varied from 100 to 700 K, the pressure from 10(-6) to 10(5) Pa.