Changes in regulation of oxidase activity of peripheral blood granulocytes in women with habitual abortions.

Generation of active oxygen forms by blood granulocytes was studied in women with a history of habitual abortions (2-3 spontaneous abortions in the first trimester, undeveloped pregnancies). The level of spontaneous luminol-dependent chemiluminescence of nonfractionated peripheral blood was increased in this patient population (study group) in comparison with women with normal reproductive function (reference group). The two groups differed by the level of activation of respiratory burst induced by opsonized zymosan and by activity of isolated granulocytes in response to chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (1-50 microM).

[Modification of method for determination of medium-molecular peptides and its application in pediatric surgery].

Taking into account clinical significance of determination of medium-molecular peptides' concentration in various surgical diseases and limitation of blood sampling in children, modification of the method for determination of medium-molecular peptides in blood serum is proposed, which requires 0.1 ml of serum and is based on deproteinization of cooled ethanol followed by spectrophotometry of peptide solution at 210 nm. Comparison study of the results of examination by this method and routine one was carried out.

[Value of individual evaluation of the responsiveness of the granulocyte system in an experiment and in clinical practice].

The paper summarizes experimental and clinical findings of neutrophilic responsiveness and the expediency of its measurements by using functional loading tests and the law of initial values. A concept of neutrophilic responsiveness phases at the cellular and tissue levels, the typology of responses to various factors, and the need to individualize the objects of pathophysiological investigations are discussed.