Evaluation of Analytes Characterized with Potential Protective Action after Rat Exposure to Lead.

Lead (Pb) was revealed for its role as a neurodevelopmental toxin. The determination of neurotransmitters (NTs) in particular brain regions could ameliorate the precise description and optimization of therapeutic protocols able to restore the harmony of signaling pathways in nervous and immune systems. The determination of selected analytes from the group of NTs based on the liquid chromatography (LC)-based method was carried out to illustrate the changes of amino acid (AA) and biogenic amine (BA) profiles observed in chosen immune and nervous systems rat tissues after Pb intoxication.

[Neurotransmitter disturbances in some parts of the rat brain and their correction under chronic and intermittent alcohol intoxication].

The pool of key neuromediators and some neurotransmitter amino acids in cerebellum, hypothalamus and midbrain of rats exposed to chronic and different variants of interrupted alcohol intoxication was investigated. The most pronounced changes were recorded in midbrain. Chronic alcohol intoxication caused an increase in the concentrations of tyrosine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), noradrenaline, tryptophan, serotonin, GABA and aspartate in this part of the rat brain.

[Assessment of the effects of chronic administration of zinc aspartate and zinc sulfate on amino acid metabolism indicators in tissues and the morphology of small intestine in young rats].

Enteral administration (every day, 10 times) of zinc aspartate (33 mg/kg) or zinc sulfate (25 mg/kg) to young rats weighing 50 - 60 g induces an amino acid imbalance in the blood plasma, liver, and myocardium. Zinc sulfate causes much more pronounced changes in the amino acid balance in the liver and myocardium, as compared to that in rats treated with zinc aspartate. One possible mechanism of the above changes can be the effect of zinc salts on nutrient absorption processes and metabolic features of zinc cations in the small intestine.

[Effect of infliximab on amino acid content in blood lymphocytes of alcohol-intoxicated rats].

Dose-dependent effects of monoclonal antibodies to tumor necrosis factor alpha (TNFa) in the form of infliximab preparation have been studied in Wistar rats upon with alcohol intoxication for 10 weeks (Lieber- De Carli liquid diet). It is established that the intraperitoneal administration of infliximab within the last 10 days of alcoholization in doses of 1 mg/kg and 10 mg/kg leads to dose-dependent changes in individual indices of the free amino acids pool and the amino acid balance in blood lymphocytes. Infliximab administered on the background of alcohol intoxication increases the pool of free amino acids and activates their metabolism in rat blood lymphocytes, which is probably due to inactivation of TNFalpha and adaptive changes in the amino acid transport system.

[Effect of tritarg on proteinogenic amino acid spectrum in blood serum and lymphocytes].

Single intragastric injection of tritarg leads to different multidirectional changes in the concentration of free proteinogenic amino acids in the blood serum and lymphocytes isolated from the blood and liver. Changes in the amino acid stock of blood and liver lymphocytes were observed 3 and 24 hours after the drug injection. The changes in concentrations of individual free amino acids are more pronounced in liver lymphocytes than in blood lymphocytes.