Publications by authors named "V M Lisak"

Young semi-domesticated pigeons captured or hatched from eggs gathered in Bratislava during 1989-1991 were examined for complement fixing antibodies to Chlamydia psittaci and agglutinating antibodies to Coxiella burnetii. Antibodies to Ch. psittaci were present in 76% of birds younger than 24 h, in 47.

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The prevalence of Coxiella burnetii and Chlamydia psittaci antibodies was investigated in 530 dog specimens divided into six groups, i. e. A = private watch dogs, B1 = service dogs from Bratislava, B2 = service dogs from other localities of Slovakia and Moravia, C = watch dogs from farms, I = household dogs, T = stray dogs.

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In 1986 and 1987 in six districts and 22 localities of the Southern Bohemian region, and in one district and one locality of the Western Bohemian region the total of 747 head of cattle was examined by a microagglutination method (MAR) and complement fixation method (KFR) for antibodies to Coxiella burnetii and Chlamydia psittaci. The positivity of C. burnetii detection was 44.

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Ring-shaped particles of 5-10 mm in diameter considered by research workers to be a slowly-sedimenting hemagglutinin (SSHA) of flaviviruses (an antigen immunologically related to virions) were detected in the precipitation band formed in immunoelectrophoresis by the non-virion ("soluble") antigen but not in the precipitation band formed by the virion antigen. The slowly sedimenting (SS) virions of tick-borne encephalitis virus previously found in a SSHA fraction did not differ in the set of structural proteins from virions of the main population (rapidly sedimenting). It is concluded from the foregoing that the hemagglutinating activity of the SS-structures is realized not by a hypothetic SSHA ("natural" ring-shaped fragment of virion envelope, precursor or a by-product of virus morphogenesis, according to other workers) but by SS-virus particles.

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