[Kinetic regularities of the interdiffusion process in a water-biopolymer system].

Kinetics of the process of interdiffusion in the system water-biopolymer has been investigated. Optical interference micromethod have been used to determine the interdiffusion coefficients in water-gelatin system. The average meanings of Dv for the water-gelatin system with gelatin concentration of 0-25% and temperature range of 293-328 K are 0.

[Enzymatic hydrolysis of gelatin].

The process of gelatin hydrolysis by means of enzymes of the proteolytic action with the aim to determine most effective destructor of gelatin macromolecules for recovering permeability and selective properties of ultrafiltration membranes was investigated. The presence of free alpha-NH2-groups was determined by means of the Lee and Takahashi method. Calculation of the destruction degree of substances in the Lee and Takahashi method during determination of the quantity of free alpha-NH2-groups rose precision of the method by 6-8%.

[Characteristics of a beta-galactosidase substance obtained by an ultrafiltration method].

The ultrafiltration method with application of Vladipor membranes YAM-300 has been used to obtain 8.9 times purified preparation of beta-D-galactosidase (EC 3.2.

[The use of ultrafiltration for concentration and purification of beta-galactosidase].

A possibility of concentration and purification of culture fluid of Escherichia coli by the ultrafiltration technique using Soviet cellulose acetate membranes were studied. It is established that cellulose acetate membranes may be used for purification from low molecular weight protein and concentration of preliminarily purified culture fluid of Escherichia coli. Membranes YAM-300 are most preferable.