Fiber Probe for Simultaneous Mid-Infrared and Fluorescence Spectroscopic Analysis.

A multispectral fiber optic probe has been developed that enables simultaneous analysis of various liquid and solid samples using attenuated total reflection mid-infrared spectroscopy and fluorimetry. The probe design was optimized using ray-tracing simulation of the light propagation. Technical evaluation of the probe has confirmed its output signal quality that was comparable to that of respective probes for single methods.

Synergy Effect of Combined Near and Mid-Infrared Fibre Spectroscopy for Diagnostics of Abdominal Cancer.

Cancers of the abdominal cavity comprise one of the most prevalent forms of cancers, with the highest contribution from colon and rectal cancers (12% of the human population), followed by stomach cancers (4%). Surgery, as the preferred choice of treatment, includes the selection of adequate resection margins to avoid local recurrences due to minimal residual disease. The presence of functionally vital structures can complicate the choice of resection margins.

Evaluation of Discrimination Performance in Case for Multiple Non-Discriminated Samples: Classification of Honeys by Fluorescent Fingerprinting.

In this study we develop a variant of fluorescent sensor array technique based on addition of fluorophores to samples. A correct choice of fluorophores is critical for the successful application of the technique, which calls for the necessity of comparing different discrimination protocols. We used 36 honey samples from different sources to which various fluorophores were added (-(2,2'-bipyridyl) dichlororuthenium(II) (Ru(bpy)), zinc(II) 8-hydroxyquinoline-5-sulfonate (8-Ox-Zn), and thiazole orange in the presence of two types of deoxyribonucleic acid).

Synergy of Fluorescence and Near-Infrared Spectroscopy in Detection of Colorectal Cancer.

Background: Colorectal cancer is one of the most common malignancies worldwide. There is an urgent need for simple and fast methods to improve tumor detection in the diagnostic and intraoperative setting to avoid complications and provide objective information in distinguishing malignant and benign colorectal tissue. Optical spectroscopy methods have recently shown a great potential for this discrimination in different organs.

Synergy Effect of Combining Fluorescence and Mid Infrared Fiber Spectroscopy for Kidney Tumor Diagnostics.

Matching pairs of tumor and non-tumor kidney tissue samples of four patients were investigated ex vivo using a combination of two methods, attenuated total reflection mid infrared spectroscopy and fluorescence spectroscopy, through respectively prepared and adjusted fiber probes. In order to increase the data information content, the measurements on tissue samples in both methods were performed in the same 31 preselected positions. Multivariate data analysis revealed a synergic effect of combining the two methods for the diagnostics of kidney tumor compared to individual techniques.