Crystallization and preliminary X-ray crystallographic analysis of Mirabilis antiviral protein.

Mirabilis antiviral protein is a single-chain ribosome-inactivating protein purified from the tuberous root of Mirabilis jalapa L. We obtained several forms of crystals of the protein by the hanging drop vapor diffusion method, but most of these crystals were not suitable for X-ray crystallography. After refining the growth conditions, crystals of crystallographic quality were grown in 20-microliters droplets of an equi-volume mixture of 1.

Age differences in perception of risk.

Age differences in perception of risk and the likelihood of disregarding warning signs were examined. A total of 358 adults, between 18 and 85 years old, were asked to rate 12 warning labels with respect to (a) how risky it would be to ignore the warning, (b) how likely it was that they would disregard the warning, and (c) how familiar they were with the labels. The older groups indicated higher risk in ignoring the signs than did the younger group.

Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase.

The crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase complexed with a 20-amino acid substrate analog inhibitor has been solved and partially refined at 2.7 A resolution to an R factor of 0.212.

Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate.

The 2.3-A crystal structure of recombinant human dihydrofolate reductase (EC 1.5.