Cross-linking effect on dentin bond strength and MMPs activity.

Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis.

Methods: Freshly extracted molars (n=80, 20 for each group) were selected to conduct microtensile bond strength tests.

Experimental use of an acrolein-based primer as collagen cross-linker for dentine bonding.

Objectives: The objective of the present study was to investigate the long-term effect of 0.01% acrolein (ACR) aqueous solution, employed as an additional primer, on the mechanical durability and enzymatic activity of resin-dentine interfaces created with a simplified etch-and-rinse adhesive.

Methods: Dentine surfaces were etched with 35% phosphoric acid for 15s, rinsed and blot-dried.

Polyurethane foam scaffold as in vitro model for breast cancer bone metastasis.

Unlabelled: Breast cancer (BC) represents the most incident cancer case in women (29%), with high mortality rate. Bone metastasis occurs in 20-50% cases and, despite advances in BC research, the interactions between tumor cells and the metastatic microenvironment are still poorly understood. In vitro 3D models gained great interest in cancer research, thanks to the reproducibility, the 3D spatial cues and associated low costs, compared to in vivo and 2D in vitro models.