[Influence of propagating electrical signals on delayed luminescence of geranium leaves. II. Theoretical analysis].

A mathematical model of delayed luminescence was devised. It is based on the description of primary photosynthetic reactions, which takes the donor and acceptor parties of photosystem II and the electrochemical gradient on thylakoid membranes into account. The model was used for the theoretical analysis of the mechanisms of changes in delayed luminescence characteristics, induced by variation potentials.

[Influence of propagating electrical signals on delayed fluorescence of geranium leaves. 1. Experimental analysis].

It was shown that local damaging stimulation of geranium graft induced the generation and propagation of electrical signals (variation potential), which changed the parameters of delayed luminescence in the leaf blade. The responses of delayed luminescence included two stages, which probably occur by different mechanisms.

[Electrophoretic analysis (SDS PAGE) of ovulatory cervical mucus in patients with fertility failure and after unsuccessful IVF].

Objective: To evaluate the presence and activity of local sperm antibodies and electrophoretic analysis of ovulatory cervical mucus (OCM) to prove the correlation of results of Kremer mucus sperm capillary penetration test and i-MAR (mixed antiimmunoglobulin reaction) test with SDS-PAGE.

Design: Retrospective study.

Setting: Department of Obstetrics and Gynecology, Medical Faculty of Charles University, Pilsen.

Anti-phospholipid antibodies against phosphatidylinositol, and phosphatidylserine are more significant in reproductive failure than antibodies against cardiolipin only.

Problem: The humoral immune response to phospholipids was investigated in women with reproductive failure [1073 women after one in vitro fertilization (IVF), 853 women after two and more IVF, 627 women after three and more repeated spontaneous miscarriages or missed abortions, 412 women after diagnostic laparoscopy] and compared with that of 391 healthy fertile women.

Method Of Study: Sera from all women in the study were tested by enzyme-linked immunosorbent assay (ELISA) for the detection of IgG, IgA, and IgM isotypes of antibodies against seven phospholipids (aPLs), i.e.

Six kinds of anti-phospholipid antibodies (aPLs) in ovulatory mucus and seminal plasma from couples with repeated miscarriages.

Objective: Determination of aPLs in ovulatory mucus and in seminal plasma in 18 infertile couples (women, median age 33.2, range 28-39 years; men, median age 36.4, range 29-46 years) with repeated unexplained miscarriages (four to nine spontaneous abortions).