New simple liquid chromatographic method for the determination of trimethoprim, sulfadiazine and N4-acetylsulfadiazine in plasma of broilers.

A new method for simultaneous quantification of trimethoprim, sulfadiazine and N4-acetylsulfadiazine in plasma of broilers at levels down to 13-16 ng/ml has been developed. Samples were deproteinized with acetonitrile, defatted with hexane, and extracted with dichloromethane. Chromatographic analysis was carried out on a C18 column in the presence of tetrabutylammonium hydrogen sulfate, a competing base, while detection was performed at 240 nm for trimethoprim, and 270 nm for both sulfadiazine and N4-acetylsulfadiazine.

Influence of inositolhexaphosphori acid (phytic acid) on the copper distribution in tissues and the excretion of copper in rats.

The aim of the present study was to investigate the effect of the administration of phytic acid on copper (Cu) concentrations in several different rat tissues. The animals used were divided into three groups: Group A (received a diet supplemented with 2% phytic acid), group B (received a diet supplemented with 10% phytic acid) and group C (control). At the end of the experiment, the animals were sacrificed and the concentration of copper was determined in the different tissues.

Effects of cadmium chloride administration on the macroscopic and microscopic characteristics of ejaculates from Chios ram-lambs.

The effects of cadmium chloride on the volume of the ejaculate, semen density, total number of spermatozoa per ejaculate, viability, grade of motility, and morphological abnormalities were studied in 3-month-old ram-lambs of the Chios breed. Two groups of seven animals each were used. For a period of 7 months, one group was treated with a daily oral dose (3 mg/kg b.

Influence of zinc oxide and eugenol sealer on concentration of zinc, calcium and copper in rat tissues.

A zinc oxide and eugenol root canal sealer (Roth 811) and sterile saline solution were injected into the dorsal thoracic midline of 70 male Wistar-Furth rats. Every day for the next 7 days, 10 animals were sacrificed by either inhalation. The liver, heart, kidneys and brain were removed from the animals and analysed for zinc, calcium and copper concentrations by flame atomic absorption spectrophotometry.

[Effect of estradiol valerate on the male reproductive organs and various semen parameters in rats].

The effect of the administration of estradiol valerate on the male reproductive organs, on their histological structure, and several semen parameters of Wistar rats was studied. In experiment A and B 140 micrograms estradiol valerate/kg b.w.