Publications by authors named "V Kloboves-Prevodnik"
Flow cytometric (FC) immunophenotyping and T-cell receptor (TCR) gene rearrangement studies are essential ancillary methods for the characterisation of T-cell lymphomas. Traditional manual gating and polymerase chain reaction (PCR)-based analyses can be labour-intensive, operator-dependent, and have limitations in terms of sensitivity and specificity. The objective of our study was to investigate the efficacy of the Phenograph and t-SNE algorithms together with an antibody specific for the TCR β-chain constant region 1 (TRBC1) to identify monoclonal T-cell populations.
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- The Ki-67 proliferative index (PI) is crucial for diagnosing nodal B-cell lymphoma (nBCL), but its evaluation in cytological samples lacks standardization.
- This study aimed to create a consistent method for accurately measuring Ki-67 PI in cytological slides to distinguish between less aggressive (indolent) and more aggressive forms of nBCL.
- The findings indicated a strong correlation between manual and flow-cytometry counting of lymphoma B cells and established specific cut-off values for Ki-67 PI using various counting methods, effectively differentiating between indolent and aggressive nBCLs.
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- Extranodal marginal zone lymphomas (eMZL) can develop in various organs, and their mutations differ by organ type; this study focused on primary breast marginal zone lymphomas (PBMZL).
- The investigation of 15 cases (14 female, 1 male) utilized various techniques including immunohistochemistry and high-throughput sequencing, revealing specific mutations predominantly affecting the NF-κB pathway and shared similarities with other types of MZL.
- Findings showed PBMZL is mutation-driven rather than reliant on gene fusions, indicating the absence of certain translocations and pathogens like Borrelia spp. in the cohort, with one patient noted for a previous skin condition.
The withdrawal of the iView detection system (iV) forced many cytopathology laboratories, including ours, to substitute immunocytochemical (ICC) staining protocols for routine practice with other detection systems. Our objective was to optimize, validate, and implement ICC protocols using OptiView (OV) and EnVision FLEX (EnV) detection systems, comparing the results with those obtained using iV. Residual cytologic samples with known diagnoses were used, testing antibodies for the ten most common markers in routine cytopathology diagnostics (calretinin, Ber-EP4, MOC-31, CKAE1/AE3, CK5/6, CD68, LCA, desmin, HBME-1, and WT1).
View Article and Find Full Text PDFBackground: Diffuse large B-cell lymphoma, not otherwise specified (DLBCL, NOS) is the most common type non-Hodgkin's lymphoma, where the treatment of relapsed/refractory cases is the major challenge. Programmed cell death protein 1 (PD-1) and its ligand PD-L1 play a crucial role in the negative regulation of the immune response against the disease. The aim of the study was to analyze the expression of PD-1 and PD-L1 on lymphoma cells (LCs) and tumor-immune cells (TICs) and to investigate their correlation with outcome.
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