Cloning of Japanese quail ovalbumin cDNA in E. coli.

Japanese quail oviduct total cDNA was synthesized from total mRNA by the classical method, using AMV reverse transcriptase, the Klenow fragment of DNA polymerase I and S1 nuclease. The results of the synthesis of total ss-cDNA partially differed from those published for the synthesis of chicken total ss-cDNA. The presumed causes of the differences in the complete reverse transcription of mRNAcon and incomplete reverse transcription of mRNAlys and a large part of mRNAov in our case are discussed.

Isolation and partial characterization of Japanese quail oviduct mRNAs.

The total mRNA was prepared from laying quail oviduct and, after denaturation with dimethyl sulphoxide and glyoxal, characterized by agarose gel electrophoresis. The electrophoresis showed that the preparation contains physically homogeneous molecules of mRNAs, which migrate as discrete bands. The molecular weights of four major oviduct mRNAs coding for conalbumin, ovalbumin, ovomucoid, and lysozyme were determined.