Microbiota of the Mammary Gland in Wistar Rats with Chemically Induced Breast Cancer after Treatment.

We conducted a comparative study of the mammary gland microbiota in female Wistar rats and the microbiota associated with breast cancer (BC) induced by the administration of N-methyl-N-nitrosourea, after surgical treatment, photodynamic therapy (PDT), and chemotherapy (CT). Selective nutrient media and a smear-fingerprint technique were used to study the microbiota. Staphylococcus, Streptococcus, and Lactobacillus were found in the mammary glands of intact rats.

Changes in the Tissue Barrier after Exposure to Lipopolysaccharide on the Apical Side of Enterocytes and the Follicle-Associated Epithelium in Peyer's Patches of the Rat Intestine.

To study the para- and transcellular permeability of columnar epithelium and follicle-associated epithelium of Peyer's patches in the rat intestine, LPS was applied from the mucosal side to simulate the action of endotoxins from gram-negative bacteria of gut microbiota. LPS did not affect transepithelial resistance or sodium fluorescein permeability, but increased the levels of claudin-3 and claudin-4 in enterocytes, suggesting strengthening of the paracellular intestinal barrier. Transcellular permeability was evaluated by electron microscopy based on the number of vesicular structures in the cytoplasm of different cell types.

Correction: Asadian et al. Rhenium Perrhenate (ReO4) Induced Apoptosis and Reduced Cancerous Phenotype in Liver Cancer Cells. 2022, , 305.

In the original publication [...

Ultrastructural Myocardial Reorganization during Experimental Treatment with Doxorubicin and Atorvastatin.

We studied ultrastructural reorganization of the myocardium in Wistar rats treated with doxorubicin (single intraperitoneal injection in a dose of 15 mg/kg) and atorvastatin (daily intragastric administration in a dose of 20 mg/kg for 7 days) individually and in combination. It was found that doxorubicin administered alone or in combination with atorvastatin induces disturbances of varying severity in cardiomyocyte ultrastructure indicating the development of regenerative and plastic myocardial insufficiency. In case of isolated administration of atorvastatin, lytic changes in myofibrils, increased autophagic processes, and segregation of the fibrillar and granular components in the nucleoli appeared in the ultrastructure of some cardiomyocytes with increasing the observation period.

A defined road to tracheal reconstruction: laser structuring and cell support for rapid clinic translation.

One of the severe complications occurring because of the patient's intubation is tracheal stenosis. Its incidence has significantly risen because of the COVID-19 pandemic and tends only to increase. Here, we propose an alternative to the donor trachea and synthetic prostheses-the tracheal equivalent.