[Analysis of Proliferation and Survival of Agrobacteria after Inoculation of Maize Pistil Filaments].

While the authors have previously developed a method of pistil filament treatment with Agrobacterium cells during blossoming for the transformation of maize generative cells, the mechanism for bacterial T-DNA penetration into the embryo sac remained unknown. This article analyzes the possibility of agrobacterial penetration into the maize embryo via pollen tubes. Microbiological, PCR, and GUS techniques were used to confirm that agrobacteria could spread for up to 20 cm from the sie of inoculation and were detected in maize embryo tissues as aerly as 24 h after inoculation, while they were not revealed after 5-13 days.

[A STUDY OF THE ISOLATED BACTERIOPHAGE ΦAB-SP7 ADSORPTION ON THE CELL SURFACE OF THE AZOSPIRILLUM BRASILENSE SP7].

The bacteriophage ΦAb-Sp7 was isolated from the cells of the Azospirillum brasilense Sp7. The morphology, size of the gram-negative colonies, and range of lytic activity against other strains and species of the genus Azospirillum was tested. The isolated phage DNA was examined using electrophoretic and restriction analysis, and the size of the genome were established.

[Agrobacterium-mediated in Planta transformation of maize germ cells].

Abstract-A transfer DNA (T-DNA) carrying the marker gene nptII was detected in the genomes of diploid and haploid maize plants obtained after the treatment of pistil filaments with a suspension of Agrobacterium during artificial pollination. PCR analysis of total DNA isolated from 155 canamycin-resistant diploid F1 seedlings revealed T-DNA insertions in the genomes of 111 plants (32.7% of the total number of analyzed seeds).

[Genetic divergence of Vipera berus and Vipera nikolskii (Reptilia: Viperidae, Vipera) populations in lower Volga and adjacent territories assessed according to the sequences of cytochrome oxidase III 12S ribosome RNA genes].

The nucleotide sequences of mitochondrial genome fragments containing the genes coding for cytochrome oxidase subunit III (COIII) and 12S ribosomal RNA of common European viper and Nikolsky's viper from various habitats (Saratov, Samara, and Penza oblasts; Chuvash Republic; and the Republic of Mordovia) were determined. According to the sequencing data, all samples clustered into two groups except for a number of individuals carrying single mutations in the genes in question. One group comprised V.

[In planta transformation of maize through inoculation of Agrobacterium into the silks].

Integration of T-DNA into the maize genome as a result of treatment of silks with Agrobacterium cells, containing activated vir genes, was demonstrated. In planta treatment of maize (Zea mays L) was performed during flowering in field. Cell suspension of Agrobacterium tumefaiciens strain GV3101(pTd33), carrying activated vir genes, was applied onto the previously isolated silks, which were afterwards pollinated with the pollen of the same cultivar.