Effect of temperature and oxygen on luminescence spectra and polarization of divinylbenzoxazolylbiphenyl thin films.

H-type molecular aggregation as assembly with chromophore dipoles arranged parallel to each other has been observed in absorption and luminescence spectra of divinylbenzoxazolylbiphenyl thin films deposited on quartz glass substrate by thermovacuum method. The reversible changes of fluorescence anisotropy have been observed under film heating below the glass transition temperature correlating with lifetime changes. The addition of oxygen was found to cause an essential luminescence quenching.

Isotopically coded cleavable cross-linker for studying protein-protein interaction and protein complexes.

An emerging approach for studying protein-protein interaction in complexes is the combination of chemical cross-linking and mass spectrometric analysis of the cross-linked peptides (cross-links) obtained after proteolysis of the complex. This approach, however, has several challenges and limitations, including the difficulty of detecting the cross-links, the potential interference from non-informative "cross-linked peptides" (dead end and intrapeptide cross-links), and unambiguous identification of the cross-links by mass spectrometry. Thus, we have synthesized an isotopically coded ethylene glycol bis(succinimidylsuccinate) derivate (D12-EGS), which contains 12 deuterium atoms for easy detection of cross-links when applied in a 1:1 mixture with its H12 counterpart and is also cleavable for releasing the cross-linked peptides allowing unambiguous identification by MS sequencing.

[Criteria for forensic medical evaluation of professional working capacity loss].

The main and additional criteria used in evaluation (in percent) of loss of professional working capacity are characterized. Criteria common for forensic medical and medical social expert evaluations and differences between them are discussed. These differences are due to the fact that forensic medical expert evaluations are based on the Civil and Civil Processual Codes of the Russian Federation but not on the departamental norm-setting documents.

[Organization and procedure of forensic medical expert evaluation of professional working capacity loss].

Sixty-one forensic medical expert conclusions on the loss of professional working capacity are analyzed and typical errors are characterized. The procedure of forensic medical expert evaluation of the degree of loss of professional working capacity in accordance with the Civil Code and Civil Judicial Procedure Code of the Russian Federation and the actual norm-setting documents is described.