[Kinetics of protein hydrolysis during the induced autolysis of the Saccharomyces cerevisiae biomass].

The kinetics of the initial stages of autolysis of the yeast Saccharomyces cerevisiae was studied and the following kinetic parameters were determined: induction period, steady-state rate of the enzyme-induced effect, and steady-state concentration of intermediate products. It was shown that appropriate inducers were able to cause a more than fourfold increase in the rate of reactions catalyzed by proteinases and almost a tenfold increase in the concentration of intermediate peptides. The mean length of the peptide molecules formed in the process was determined and dependence of this value on the degree of hydrolysis was demonstrated.

[The effect of dietary fiber on proteolysis].

In the presence of food fibers, proteolysis occurs with the formation of sorption complexes. At the initial stages of hydrolysis, the adsorption of both casein and pepsin is observed, the adsorption influencing the initial rate of hydrolysis. The composition of the sorption layer changes during hydrolysis in favor of intermediates.

[Effect of enteral feeding on protein metabolism in patients with peritonitis].

Thirty patients with peritonitis of different etiology were examined. Deep alterations of protein metabolism were revealed resulting from dyscoordination of amino acid metabolism. Efficiency of unbalanced amino acid mixtures was shown and on the contrary, the absence of a therapeutic effect when using balanced mixtures.

Kinetic description of proteolysis. Part 4. Hydrolysis kinetics of partial protein hydrolysates.

This report presents experimental data on kinetics of casein hydrolysate hydrolysis. The experiments were interpreted in the framework of a theory reported, which is guided by an experimentally measurable value--a total molar concentration of all peptides and amino acids (total amino nitrogen). The total amino nitrogen N was determined by photometry of N-trinitrophenyl (N-TNP) derivatives obtained by means of trinitrobenzenesulfonic acid (TNBS).

[Coordination immobilization of fungal proteinases].

The possibility of coordination immobilization of fungal enzymes from Aspergillus niger MB and Aspergillus flavipes on the styrene copolymer with maleic acid has been explored. Optimal conditions for preparing insoluble chromium-containing enzyme polymeric complexes have been studied. The synergistic effect of salts of different metals on stability of chromium-containing immobilized preparations has been demonstrated.