Effect of chalone system on proliferation of Ehrlich ascitic tumor cells under conditions of modified photoregimen.

We studied the influence of different photoregimens (light/darkness alternation and constant darkness) on the effect of saline and chalone regulation of cell proliferation in Ehrlich ascitic tumor was studied. Saline slightly inhibited proliferation of tumor cells. Chalone-containing preparations from animals exposed to light/darkness alternation and to constant darkness were characterized by different effects on cell proliferation in this tumor.

Determination and characterization of chloroguaiacol conjugates in fish bile by HPLC.

In this study a small-scale technique for direct analysis of metabolic conjugates of 4,5,6-trichloro- (CG-3) and tetrachloroguaiacols (CG-4) in fish bile by RP-HPLC is presented. Only one metabolite, glucuronic acid conjugate, was demonstrated in two Lake Baikal fishes (Leuciscus leuciscus baikalensis and Cottus kessleri) exposed to CG-3 or CG-4 at 6 degrees C for 1-2 days. In Leuciscus the ratio between free CG-4 in the ambient water and conjugated CG-4 in the bile averaged 264,000.