Immunogenicity of recombinant vaccinia viruses expressing hepatitis B virus surface antigen in mice.

The ability of different recombinant vaccinia viruses (RVV) expressing hepatitis B virus surface antigen (HBsAg) to induce anti-HBs and anti-vaccinia virus responses has been analyzed in mice. The RVVs tested differed with regard to the original vaccinia virus strain used as vector and the site of insertion of a foreign gene. It was found that the immunological responses to RVVs based on the WR strain were higher than those to RVVs based on the Lister strain.

Complementation of cell and serum growth factors during proliferation of tumour cells in semisolid medium.

Foetal bovine serum (FBS) and the preparation Ultraser (LKB, Sweden) contain more growth factors (GF) necessary to promote proliferation of cells in soft agar than the sera from newborn and adult animals. It was found that non-dialysable GFs are produced during growth of cells in semisolid medium. The dependence of colony-forming efficiency (CFE) on plating cell dose (PCD) was studied in 11 cell lines at varying concentrations of FBS.

[Properties of viruses isolated from human fibroblasts culture treated by paraganglioma culture filtrate].

In the human fibroblasts culture, transformed by the material from malignant paraganglioma culture, there was found the RNA- and DNA containing virus synthesis. The RNA-containing virus with a buoyant density of 1.16--1.