Nanoscale size impact of nanoparticle interaction and activity studies with urease.

Herein, we provide insights into the size-dependent interactions of silver nanoparticles (AgNPs) with urease and their implications for enzyme inhibition. AgNPs with a size of 5 nm exhibited the strongest binding affinity of 66 nM, resulting in significant enzyme attachment, interfering enzyme conformation, and a consequent loss of activity. Mid-sized AgNPs, , 20 and 50 nm, exhibited binding affinities of 712 and 616 nM, causing only slight structural alterations.

Efficacy of Antimicrobial Peptide GH12 on a Multispecies Endodontic Biofilm Model: An In-vitro Study.

Objective: This study aimed to evaluate the antibacterial efficacy of different concentrations of GH12 on a simulated multispecies biofilm comprising Enterococcus faecalis, Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis.

Methods: Single rooted teeth were decoronated, cut into 1.5 mm sections to obtain dentine discs which were randomly allocated into five groups: (n=12 each), Group 1: Phosphate Buffered Solution (PBS) - negative control, Group II: 5% Sodium hypochlorite (NaOCl) - positive control, Group III: Minimum Inhibitory Concentration (MIC) of GH12, Group IV: 2x MIC of GH12, Group V: 4x MIC of GH12.

Nanocatalysis of silver-nanobioprobe based supersensitive electrochemical detection of Salmonella serotypes targeting virulence protein.

Herein, we report a supersensitive and specific detection of Salmonella employing nanocatalysis of silver nanoparticle (AgNp). A nanobioprobe was developed employing specific antibody (Ab) that binds to a peptide present in transmembrane protein of Salmonella. We have studied 7 surface-exposed peptide hits from conserved virulence proteins (PagC, ST50, PagN, CdtB and FliC).

Dynamic Dance of Chirality and Morphology: Interplay of Solvent-Sensitive Self-Assembly in Topological Evolution and Chirality Amplification.

The building block has been designed and synthesized, which exhibits different achiral morphologies in good solvents, forming nanospheres in THF and nanoflowers in 1,4-dioxane. In the presence of water as a poor cosolvent, demonstrates an agnostic behavior, generating left-handed superhelices in the water:THF (80:20) solvent system. However, when the good solvent is switched to 1,4-dioxane, a change in chirality is observed in the water:1,4-dioxane (30:70) solvent system, resulting in the formation of fused nanospheres.