[The enzymes of blood plasma and hepatic tissue in children are dependent on stage of transition to chronic state of viral hepatitis].

The detection of activity of enzymes in hepatic tissue and whole blood in children aged 12-16 years with chronic viral hepatitis was carried out. The results of study demonstrated that the level of metabolic parameters during the third stage of transition of disease to the chronic state as compared with the second stage testifies the presence of deeper functional affection of hepatic cells and more expressed metabolism disorders. The character of ratio of levels of activity of enzymes in liver and blood of children under the second and third stages of transition of disease to chronic state was analyzed.

[The activity of enzymes in lymphocytes of children and adults with chronic viral hepatitis B and C].

It is established that in patients with chronic viral hepatitis B and C the intensity of reaction is increased in lymphocytes of children as compared with adults. The study also established the limitation of turning out of substrates for macromolecular synthesis and the decrease of activity of reactions of tricarbonic acid cycle at the expense of decreasing of substrates flow from amino-acid metabolism included. Under chronic hepatitis C, in lymphocytes the levels of activity of enzymes involved in functioning of main metabolic paths and glutathione system of antioxidant defense of are decreased in children as compared with adults.

The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response.

Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on the oceanic carbon cycle, there have been a number of studies focused on how diatoms respond to limiting macro and micronutrients such as iron and nitrogen.

Examination of microbial proteome preservation techniques applicable to autonomous environmental sample collection.

Improvements in temporal and spatial sampling frequency have the potential to open new windows into the understanding of marine microbial dynamics. In recent years, efforts have been made to allow automated samplers to collect microbial biomass for DNA/RNA analyses from moored observatories and autonomous underwater vehicles. Measurements of microbial proteins are also of significant interest given their biogeochemical importance as enzymes that catalyze reactions and transporters that interface with the environment.

Iron conservation by reduction of metalloenzyme inventories in the marine diazotroph Crocosphaera watsonii.

The marine nitrogen fixing microorganisms (diazotrophs) are a major source of nitrogen to open ocean ecosystems and are predicted to be limited by iron in most marine environments. Here we use global and targeted proteomic analyses on a key unicellular marine diazotroph Crocosphaera watsonii to reveal large scale diel changes in its proteome, including substantial variations in concentrations of iron metalloproteins involved in nitrogen fixation and photosynthesis, as well as nocturnal flavodoxin production. The daily synthesis and degradation of enzymes in coordination with their utilization results in a lowered cellular metalloenzyme inventory that requires ∼40% less iron than if these enzymes were maintained throughout the diel cycle.