Chinese hamster ovary cell line DXB-11: chromosomal instability and karyotype heterogeneity.

Background: Chinese hamster ovary cell lines, also known as CHO cells, represent a large family of related, yet quite different, cell lines which are metabolic mutants derived from the original cell line, CHO-ori. Dihydrofolate reductase-deficient DXB-11 cell line, one of the first CHO derivatives, serves as the host cell line for the production of therapeutic proteins. It is generally assumed that DXB-11 is identical to DUKX or CHO-DUK cell lines, but, to our knowledge, DXB-11 karyotype has not been described yet.

[Karyotypic variability of human myeloma cell lines].

Cytogenetic analysis of human myeloma cell lines L363, Karpas 707, RPMI 8226 and U-266 has been performed. Chromosome numbers retained near-diploid (L363, Karpas 707 and U-266) or increased to hypotriploid (RPMI 8226) during many years of maintenance of the cell lines in vitro. Based on G-banding analysis, the complex karyotypes with abnormalities of virtually all chromosome pairs were found in these cell lines but common chromosome translocations were not observed.

[Metaphase chromosomes of human multiple myeloma cells of line U-266: karyotype and morpho-functional characteristics of nucleolar organizer regions].

Human multiple myeloma (MM) cell lines are widely used to investigate chromosome rearrangements typical for this disease. However, during cell cultivation both numeral and structural chromosome rearrangements usually take place in addition to changes of structural and functional status of particular chromosome regions. We investigated karyotype and morpho-functional status of nucleolar organizer regions (NORs) in human cell line MM U-266.