Crystal structure and Hirshfeld surface analysis of bis-(μ-4--but-oxy-4-oxobut-2-en-2-olato)bis-[(4--but-oxy-4-oxobut-2-en-2-olato)ethano-lzinc(II)].

The mol-ecular and crystal structure of the title binuclear Zn complex, [Zn(CHO)(CHOH)], with enolated anionic -butyl-aceto-acetate and ethanol was analysed. The coordination polyhedra of the Zn atoms are distorted octa-hedra formed by six oxygen atoms that belong to three ligand mol-ecules and a coordinated ethanol mol-ecule. In the crystal phase, alternating layers can be distinguished parallel to the plane.

Inhibition of heat-induced protein aggregation by zirconium phthalocyanines.

Specific proteins found in food sources tend to aggregate into fibrils under heat treatment; studying these aggregation processes and developing tools to control protein heat-induced aggregation is an active area of research. Phthalocyanine complexes are known to exhibit antiprionic and anti-fibrillogenic activity. Thus, the anti-fibrillogenic effect of a series of Zr phthalocyanines with different out-of-plane coordinated ligands, namely positively charged (PcZrLys ), negatively charged (PcZrCitr ), and group able to form disulfide bridges (PcZrS ), on the heat-induced aggregation of such proteins as BLG, insulin, and lysozyme was studied.

Trimethine Cyanine Dyes as NA-Sensitive Probes for Visualization of Cell Compartments in Fluorescence Microscopy.

We propose symmetrical cationic trimethine cyanine dyes with β-substituents in the polymethine chain based on modified benzothiazole and benzoxazole heterocycles as probes for the detection and visualization of live and fixed cells by fluorescence microscopy. The spectral-luminescent properties of trimethine cyanines have been characterized for free dyes and in the presence of nucleic acids (NA) and globular proteins. The studied cyanines are low to moderate fluorescent when free, but in the presence of NA, they show an increase in emission intensity up to 111 times; the most pronounced emission increase was observed for the dyes in the presence of dsDNA and with RNA.

Modification of insulin amyloid aggregation by Zr phthalocyanines functionalized with dehydroacetic acid derivatives.

Amyloid fibrils are widely studied both as target in conformational disorders and as basis for the development of protein-based functional materials. The three Zr phthalocyanines bearing dehydroacetic acid residue (PcZr(L1)2) and its condensed derivatives (PcZr(L2)2 and PcZr(L3)2) as out-of-plane ligands were synthesized and their influence on insulin fibril formation was studied by amyloid-sensitive fluorescent dye based assay, scanning electron microscopy, fluorescent and absorption spectroscopies. The presence of Zr phthalocyanines was shown to modify the fibril formation.

Fluorescent β-ketoenole AmyGreen dye for visualization of amyloid components of bacterial biofilms.

Green-emitting water-soluble amino-ketoenole dye AmyGreen is proposed as an efficient fluorescent stain for visualization of bacterial amyloids in biofilms and the detection of pathological amyloids in vitro. This dye is almost non-fluorescent in solution, displays strong green emission in the presence of amyloid fibril of proteins. AmyGreen is also weakly fluorescent in presence to biomolecules that are components of cells, extracellular matrix or medium: nucleic acids, polysaccharides, lipids, and proteins.