Electrooptical Analysis of Microbial Cell Suspensions forDetermination of Antibiotic Resistance.

The effects of ampicillin; kanamycin, chloramphenicol, and tetracycline on electrophysical characteristics of cells of sensitive (ampicillin; kanamycin, chloramphenicol) and resistant (ampicillin; kanamycin, chloramphenicol, tetracycline) Escherichia coli strains were studied. Under the action of antibiotics sensitive and resistant E. coli strains acquire different electro-optical properties.

[Microbial degradation of components of waste water from phenol-producing industry].

Processes of aerobic biodegradation of components of phenol production sewage (phenol, acetophenone, dimethylphenylcarbinol, cumene hydroperoxide, alpha-methylstyrene, benzoate, and p-hydroxybenzoate) by bacterial strains obtained from the collection of Saratov Institute of Biocatalysis were studied. The metabolic reactions were shown to be oxidative and have a common catabolic sequence (cumene hydroperoxide-dimethylphenyl-carbinol alpha-methylstyrene-acetophenone-phenyl acetate-phenol-pyrocatechol-aromatic ring breakage). Benzoate and p-hydroxybenzoate were degraded through the formation of pyrocatechol and protocatechuate, respectively.

[Storage of industrial microorganisms entrapped into polymer matrices].

Our study of the techniques of long-term storage of the biomass of various strains of microorganisms, which cause breakdown or transformation of synthetic organic compounds, demonstrates that desiccated agar beads with immobilized microbial cells can be used for this purpose. In addition, the cells can be stored in desiccated matrices of agar or polyvinyl alcohol, coating synthetic cords. Such dry biocatalysts may be used for quick starting of bioreactors and in other biotechnological processes.

[Protective effects of agar during immobilization of strains capable to degrade aromatic compounds].

A study of utilization of toxic monoaromatic compounds by microbial cells incorporated into agar matrix revealed positive effects of immobilization on the rate and completeness of biodegradation. This increase in the degrading activity is probably due to a strong protective effect of the polysaccharide carrier. The protective properties of agar are not due to sorptional and diffusional processes in the matrix.

[Main principles of the treatment of surgical patients with concomitant diabetes mellitus].

A total of 955 surgical patients with concomitant diabetes mellitus were treated at the Stomatological Medical Institute Surgery Faculty Hospital. Therapeutic experience made physicians arrive at a conclusion on the necessity of active detection of carbohydrate metabolic derangements in high risk patients, as well as reasons for the decompensation of diabetes mellitus in surgical patients. Decompensation was caused by the activation of the hypothalamohypophyseal-adrenal system in stress and a considerable increase in contrainsular factors in the blood with a decrease in adaptive function of the pancreas.