[Biodiesel from microalgae: ways of increasing effectiveness of lipids accumulation by genetic engineering methods].

Microalgae are viewed as one of the most perspective producer of lipids for biodiesel production. The review shows the results of researches of genes' expression increase actually included in fatty acids biosynthesis. The increase of effectiveness of solar energy absorption and carbon dioxide fixation influences the microalgae productivity.

[Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation of microtubules].

The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis.

[Developing transgenic barley lines producing human lactoferrin using mutant alfa-tubulin gene as selective marker gene].

Method of biolistic transformation was used for genetic improvement of commercial barley cultivars (Oksamitoviy, Vodogray and Getman). The plasmid pHLFTuBA was used for particle bombardment that consists of the hLF gene under the control of the barley glutelin B-1 promoter and a selectable marker gene, alpha-tubulin conferring resistance to trifluralin (dinitroanilinr herbicide). Preliminary screening of different trifluralin concentration range from 0,1 to 30 microM was tested for determination of effective selective agent concentration.