[Target-directed search of anti-virulence drugs].

Modern medicine now encounters with problem of the absence of effective antibacterial drugs, which are able to render therapeutic effect on chronic form of infectious process. Thus, the actual objective is to develop essentially new generation of drugs, on the basis of which should lie identification of new bacterial targets playing key role in process of chronization of infection as well as selection of new physiologically active substances, which are able to render highly specific inhibitory effect on selected target. Solving of this objective is possible during realization of new approaches for search and design of new drugs and, first of all, during usage of bioinformatics methods, which enable to identify new biotargets, select most effective chemical compounds-inhibitors and optimize their pharmacological and pharmacokinetic properties.

[Acylhydrazones of 20-keto steroids and their transformations: I. Synthesis and properties of 1'-acyl-substituted 3'-methylandrosteno[16,17-d]pyrazolines].

Acetates of 3beta-hydroxy-3'-methyl-1'(N)-acylandrost-5-eno[16,17-d]pyrazolines bearing monothiooxamide acyl groups were synthesized during the study of approaches to the synthesis of 3'-methylandrosteno[16,17-d]azoles, promising biologically active analogues of 20-keto pregnenanes, and their properties were investigated. The cyclization of delta16-20-thiooxamidohydrazones to the corresponding heterocycles was shown to proceed under rigorous conditions and to depend partially on the nature of the oxamide grouping.

[Protease and alpha-amylase synthesis by washed cells of Aspergillus oryzae 251-90].

Regularities of protease and alpha-amylase production by washed cells of Aspergillus oryzae 251-90 were being studied. The results obtained enabled us to assume a constitutive character of the both enzymes synthesis by the given producer. Sources of carbon, nitrogen and sulphur take part in regulation of the protease production, whereas in the case of the alpha-amylase synthesis only carbon sources that are important.

[Immobilization of commercial glucoamylase on the polymer carrier].

Commercial glucoamylase was immobilized on the polymer carrier produced by the binding of 4--8 mol.% 4,4'-diamine diphenyl oxide of the maleic anhydride copolymer with N-vinyl pyrrolidone. Two types of immobilized preparations were obtained: with covalent absorption and covalent attachment of the enzyme to the polymer.