[THE METHOD OF COLPOPOIESIS IN AN URINARY BLADDER EXTROPHY].

Complex of extrophy--epispadias--a rare inborn malformation of genitourinary system, abdominal wall muscles and pelvic bones. The observation was adduced, concerning an urinary bladder extrophy in a girl in combination with vaginal agenesia after noneffective primarily--postponed closure of urinary bladder, osteotomy (in the age of 4 yrs old), with consequent ureterosygmostomy. In the patient there was conducted a reconstructive operation for vagina creation, using nonfunctioning bladder, right-sided nephrectomy for severe ureterohydronephrosis, reconstruction of anterior abdominal wall and external genitalia.

[The verification possibilities of immunochips under low content of antibodies to Core-antigen of virus of hepatitis C].

The detection of antibodies to Core-antigen of virus of hepatitis C in test-systems for solid-phase immune-enzyme analysis with low optical density can be a result not only of true availability of antibodies but an effect of nonspecific reaction of blood serum. The diagnostic possibilities of immunochips to be used in immune-enzyme analysis for verification of availability of markers of viral hepatitis C were investigated in conditions of low positive reaction of blood serum to core-antigen. It is established that immunochips and immunoblots have similar specificity concerning detection of antibodies to Core-antigen.

[Procedures for optimizing the intralaboratory quality control in mass screening for hepatitis C virus antibodies].

The paper presents data on the optimization of intralaboratory quality control of tests for hepatitis C virus antibodies by enzyme immunoassay in the mass screening scope of studies. It is concluded that the control material prepared without assistance is suitable only to assess the convergence of the results of a study. The problems in the monitoring of the reproducibility of study results and in the standardization of on-line quality control involving the construction and keeping of control charts are solved by using standardized commercial materials and a computer-based data processing system.

[Vitamin metabolism in children with insulin-dependent diabetes mellitus. Effect of length of illness, severity, and degree of disruption of substance metabolism].

Correlation between the state of vitamin metabolism and the impairments in carbohydrate, lipid and protein metabolism was studied in 35 children of 9-13 years of age with diabetes mellitus of various severity standing for up to 7 years. Deterioration of riboflavin metabolism in insulin-dependent diabetes mellitus, expressed as an increase of the vitamin excretion with urine, was augmented with prolongation of the disease duration; the deterioration was sometimes related to the value of glycemia and glucosuria, being the indicative symptom of the disease. In spite of some limitations in validity of experiments related to insufficient number of children in some groups, a decrease in excretion of 1-methyl nicotinamide with urine was detected in all the children with the comatose state, in acidoketosis and glucosuria (above 20 g/day), whereas normal content of nicotinamide coenzymes was found in erythrocytes.