Publications by authors named "V I Chechekhin"
Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, tissue renewal and regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g.
View Article and Find Full Text PDFStem and progenitor cells are characterized by peculiar mechanisms of hormonal regulation. Here we describe a protocol of analysis of hormonal cross-talk in adipose tissue derived multipotent mesenchymal stem cells (MSCs). Specifically, cells were treated by a "sensitizing" hormone/neuromediator followed by the measurement of cellular Ca2+ response to the "readout" hormone after various time intervals.
View Article and Find Full Text PDFArticle Synopsis
- Multipotent mesenchymal stromal cells (MSCs) play a crucial role in tissue repair by secreting proteins that can inhibit fibrosis development, particularly by affecting fibroblast differentiation into myofibroblasts.
- Research showed that dividing MSC secretome into subfractions—extracellular vesicles (EV) and soluble factors (SF)—enhances their antifibrotic effects in lab settings by preventing this differentiation.
- A proteomic analysis indicated that conditioned medium (CM) from MSCs contains activators of the NF-κB pathway, which helps regulate gene expression related to fibroblast function, while EV and SF are rich in factors associated with important signaling pathways, influencing therapeutic strategies involving MSCs.
Peripheral 5-HT/HTR6 axis is responsible for obesity-associated hypertension.
Biochim Biophys Acta Mol Cell Res
February 2024
Hypertension is one of the major life-threatening complications of obesity. Recently adipose multipotent mesenchymal stromal cells (MSCs) were implicated to the pathogenesis of obesity-associated hypertension. These cells amplify noradrenaline-induced vascular cell contraction via cAMP-mediated signaling pathway.
View Article and Find Full Text PDFNon-coding RNA (ncRNAs) genes have attracted increasing attention in recent years due to their widespread involvement in physiological and pathological processes and regulatory networks. The study of the function and molecular partners of ncRNAs opens up opportunities for the early diagnosis and treatment of previously incurable diseases. However, the classical "loss-of-function" approach in ncRNA function analysis is challenged due to some specific issues.
View Article and Find Full Text PDF