HIGH-THROUGHPUT SCREENING TECHNOLOGY FOR MONITORING PHYTOHORMONE PRODUCTION IN MICROALGAE(1).

New miniaturized techniques for multiplying microalgae and estimating their phytohormone production were developed; in these methods, the strains to be tested are cultivated in microtitre plates, and the phytohormones in suspensions of the cultures are measured by direct ELISAs. Specific and sensitive ELISAs for determining abscisic acid (ABA), indole-3-acetic acid (IAA), cis- and trans-zeatin riboside, isopentenyladenosine (iPR), and other less common cytokinins were developed for this purpose. Polyclonal antibodies used in the ABA and IAA assays were raised against C1- and C1'- conjugates of the compounds with BSA, respectively, and thus were specific for the free acids and their respective C1-derivatives.

Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography-mass spectrometry.

Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an immunoaffinity chromatography (IAC) gel. The detection limit of the ELISA was approximately 4.6x10(-10)mol/L.

[Therapeutic and cosmetic effect of cryocoagulation therapy of basaliomas in the area of the eye].

The authors evaluated the results of cryosurgical intervention in a group of patients with advanced basaliomas with surgically adverse localizations from the aspect of relapses and the cosmetic effect. Due to the simple procedure and achieved effect this technique is a considerable asset.