Publications by authors named "V Hrabal"
Article Synopsis
- - TP73, part of the TP53 gene family, produces different protein variants (TAp73 and ΔTAp73) with opposing functions through various genetic mechanisms.
- - Newly developed antibodies for these p73 variants reveal that TAp73 is present in multiciliated epithelial cells, while ΔTAp73 marks non-proliferative basal cells in squamous epithelium.
- - In cervical squamous cell carcinomas, p73α is commonly expressed and linked to lower tumor grades, whereas TAp73 appears less frequently and does not show significant associations with cancer characteristics.
Article Synopsis
- Monoclonal antibodies that target immune checkpoints are changing cancer treatment, but their effectiveness varies and can lead to unexpected issues like hyperprogression.
- Current animal research models, especially mice, don’t accurately reflect the human immune system and patient differences, creating a need for better models.
- This study introduces two new antibodies that effectively target canine PD-1, offering valuable tools for canine cancer research and potential new treatments for dogs with cancer.
Interferon induced transmembrane proteins (IFITMs) play a dual role in the restriction of RNA viruses and in cancer progression, yet the mechanism of their action remains unknown. Currently, there is no data about the basic biochemical features or biophysical properties of the IFITM1 protein. In this work, we report on description and biochemical characterization of three conformational variants/oligomeric species of recombinant IFITM1 protein derived from an expression system.
View Article and Find Full Text PDFThe gene encodes two major protein variants; TAp63 contains a p53-like transcription domain and consequently has tumor suppressor activities whereas ΔNp63 lacks this domain and acts as an oncogene. The two variants show distinct expression patterns in normal tissues and tumors, with lymphocytes and lymphomas/leukemias expressing TAp63, and basal epithelial cells and some carcinomas expressing high levels of ΔNp63, most notably squamous cell carcinomas (SCC). Whilst the transcriptional functions of TAp63 and ΔNp63 isoforms are known, the mechanisms involved in their regulation are poorly understood.
View Article and Find Full Text PDFHDMX and its homologue HDM2 are two essential proteins for the cell; after genotoxic stress, both are phosphorylated near to their RING domain, specifically at serine 403 and 395, respectively. Once phosphorylated, both can bind the p53 mRNA and enhance its translation; however, both recognize p53 protein and provoke its degradation under normal conditions. HDM2 has been well-recognized as an E3 ubiquitin ligase, whereas it has been reported that even with the high similarity between the RING domains of the two homologs, HDMX does not have the E3 ligase activity.
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